PBSerum Facial Certification

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Recap on Skin Aging and Physiology

As professionals in skin care we have already learned that physiology is the science that treats the functions of the living organism and its parts, and the physical and chemical factors and processes involved. It refers to all the normal functions that take place in a living organism.

Cell physiology is the biological study of phenomena involved in cell growth, maintenance, self-regulation and division of cells.

HUMAN SKIN STRUCTURE

An organ is a collection of different tissues which have a particular organization and perform one (or several) common function. The skin is the largest organ of the human body. It accounts for 15% of the total adult body weight and measures about 20 square feet in surface area. It forms the outer covering for the entire body and protects the internal tissues from the external environment.

The skin has many vital functions. The principal functions of the skin are the protection of the underlying structures; excretion, secretion, absorption, pigmentogenesis, accumulation, sensory perception, thermoregulation, and regulation of immunological processes.  The skin is also an agent for the supply of vitamin D through the effect of sunlight, which is essential for the growth and maintenance of our bones. The skin does not have any blood vessels within it. Most of the skin can be classified as thin skin. Thick skin is found only on the palms of the hands and the soles of the feet.

These functions are all affected by the structural changes in the skin with ageing and, after middle age, most functions are reduced, some by as much as 50 – 60%. Much importance is attached to the appearance of our skin, especially in our modern society.

The skin consists of three layers: The epidermis, the dermis and the hypodermis.

The epidermis, the outermost layer of skin, provides a waterproof barrier and creates our skin tone. It consists of a specific constellation of cells known as keratinocytes, which function to synthesize keratin, a long, threadlike protein with a protective role. The normal epidermis is a stratified squamous epithelium undergoing continuous renewal. The major cell in the epidermis is the ectodermally derived keratinocyte, making up approximately 95% of the epidermal cells.

The epidermis is a thin layer of skin. It is the most superficial layer of skin, the layer you see with your eyes when you look at the skin anywhere on your body. Functions of the epidermis include touch sensation and protection against microorganisms.

This skin is further divided into five, separate layers. In order from most superficial to deepest, they are the:

  • Stratum Corneum
  • Stratum Lucidum
  • Stratum Granulosum
  • Stratum Spinosum
  • Stratum Basale

The dermis, beneath the epidermis, contains tough connective tissue, hair follicles, and sweat glands. This layer is fundamentally made up of the fibrillary structural protein known as collagen. These protein reduces with age and contributes to the more fragile skin of elderly people. Medications also weaken the collagen fibers and cause thinning of the skin in the long term.

The dermis lies on the subcutaneous tissue, or panniculus, which contains small lobes of fat cells known as lipocytes.

The dermis contains:

  • Blood vessels that nourish the skin with oxygen and nutrients. The blood vessels also allow immune system cells to come to the skin to fight an infection. These vessels also help carry away waste products.
  • Nerves that help us relay signals coming from the skin. These signals include touch, temperature, pressure, pain, and itching.
  • Various glands.
  • Hair follicles.
  • Collagen, a protein that is responsible for giving skin strength and a bit of elasticity.

The hypodermis, the deeper subcutaneous tissue is made of fat and connective tissue. This layer is not truly part of the skin itself but connects the skin loosely to the underlying muscles and bones that make up the deeper tissues of the body. The layer of fat here acts as a cushion against physical trauma to internal organs, muscles, and bones.

This following image details the parts of the integumentary system.

THE STRUCTURE OF HUMAN SKIN CELLS

You may have noticed that when your skin is dry, it gets flaky and pieces fall off. Skin cells die, slough off, and are replaced by new skin cells. The process slows down as you get older, but it never stops. A cell is a structure as well as a functional unit of life.

Within the epidermis are layers of four different kinds of skin cells: keratinocytes, melanocytes, Merkel cells, and Langerhans cells.

Keratinocytes are the most common among skin cells. They account for between 90% and 95% of your skin. Keratinocytes produce the protein keratin, and by the time the cells have been pushed up from the basement membrane, they are mostly sacks filled with keratin.     

This protein is structural and provides your outer skin with its strength and helps it act as a barrier. It gives skin its resistance and makes it waterproof. Nails and hair are made of keratin, that’s why you might see beauty products advertise it as an ingredient.

Melanocytes – Melanin is the pigment that gives your skin its color, and it is produced in specialized skin cells called melanocytes. The darker your skin is, the more melanin these cells produce. They can be found deep in the epidermis, close to the basement membrane.

Merkel Cells are found in the basal layer of the epidermis and the epithelial sheath of hair follicles. Merkel cells are associated with sensory nerve endings in the skin and may function as mechanoreceptors.

Langerhans cells are antigen-presenting cells derived from bone marrow. They make up 3% to 6% of all cells in the epidermis and are found mainly within the spinous layer. With aging and chronic sun exposure, the number of Langerhans cells decreases.

This following image details the structure of human skin cells.

SKIN TYPES

Understanding the different skin types: normal, dry, oily, acne and combination

Normal skin is a well-balanced skin. The scientific term for healthy skin is eudermic. Normal skin is neither too oily not too dry.

Identification:

  • Fine pores
  • Good blood circulation
  • Soft and smooth texture
  • Fresh, rosy color
  • No blemishes

Dry skin is a skin type that produces less sebum than normal skin. As a result of the lack of sebum, dry skin lacks the lipids that it needs to retain moisture and build a protective shield against external influences. Dry skin looks dull and can feel tight and rough. As a result, the skin’s barrier function can become compromised.

Identification:

  • Dry appearance
  • Brittle and rough
  • Dull
  • Elasticity is low

Oily skin is a skin type with heightened sebum production. This over production is known as seborrhea. Oily skin has a shine and pores are visible. Oily skin is prone to comedones (blackheads and whiteheads) and to the varying forms of acne. For oily skin it is essential to follow a daily skin care routine with appropriate products.

Identification:

  • Enlarged, clearly visible pores
  • Glossy shine
  • Blood vessels may not be visible
  • Pale skin

Acne Skin occurs when sebaceous (oil) glands attached to the hair follicles are stimulated at the time of puberty or due to other hormonal changes. Without treatment, dark spots and permanent scars can appear on the skin as acne clears.

Identification:

  • Blackheads
  • Whiteheads
  • Pustules (bumps containing pus)
  • Tender red bumps also known as pimples
  • Cysts (deep pimples or boils)
  • Nodules

Combination skin consists of a mix of skin types that vary between the T-zone and the cheek area.

Identification:

  • Oily T-zone (forehead, chin and nose)
  • Enlarged pores in this area
  • Normal to dry cheeks

 

SKIN COLOR

Human skin comes in a wide variety of colors, ranging from shades of dark brown to almost white. Although an individual’s skin color is influenced by numerous factors, the most significant is its content of a pigment called melanin.

Some people make more melanin than others. Everyone has about the same number of cells that make melanin, but not everybody makes the same amount of melanin.

The more melanin your skin makes, the darker your skin. How much melanin your body makes depends on your genes, which you get from your parents.

Melanin is why you get a tan or burn. When you go out in the sun, your body makes more melanin. That’s because it’s trying to protect you from the sun’s damaging rays by deflecting or absorbing them. But melanin isn’t strong enough to completely protect you, especially if you have pale skin. By the time you see your skin turn darker or get burned, it’s already damaged, so trying to get a tan is a bad idea. You should always protect your skin from the sun by covering up and using sunscreen.

Because people with darker skin have more melanin, they usually don’t get as wrinkly when they get older. They’re also less likely to get skin cancer. But even dark-skinned people can get wrinkles and skin cancer, so everyone needs to protect their skin when they go outside.

This following image details cross-section of skin showing melanin in melanocytes

The Fitzpatrick system of classifying skin type is most commonly used in the assessment of skin cancer risk.

 

 

 

 

SKIN AGING

Many factors cause the skin to age. One factor is genetic makeup which fortunately, some of the factors contributing to biological aging can be controlled. A healthful diet full of antioxidant-rich fruits and vegetables, along with nutritional supplementation and topical application of key nutrients, may help decrease the intensity and delay the onset of many changes that are a result of biological aging. Water is an essential component for the effective functioning of our body. Water is essential to maintain the optimum skin moisture and deliver essential nutrients to the skin cells. It replenishes the skin tissue and increases its elasticity. This also helps delay the appearance of signs of ageing like wrinkles and fine lines.

Another aging causing factor is the result of continually repeated muscle movements. Repeated muscle movements day after day for years causes wrinkles. Repetitive muscle contractions cause stress on the skin, repeated wrinkle causing behaviors can also break down collagen. Squinting, pursing the lips while smoking, drinking from a straw, resting chin or cheek on the hand and sleeping on your side or stomach are things that should be avoided to help prevent premature signs of aging.

Sun exposure is the primary environmental stressor that causes premature skin. UV rays can penetrate the skin and cause damage to collagen producing fibroblast cell. Other pollutants like cigarette smoke, exposure to harsh weather conditions like dry air, wind, and cold and pollution are great contributors to environmental aging. UV damage from the sun accounts for 90% of premature skin aging.  Photoaging damages collagen, melanocytes, elastin and the skin moisture barrier.

Improper skin care is another aging factor. Using harsh cleansers and neglecting to supplement the skin with rich, nourishing moisturizers.

As you age, your melanocytes (pigment cells) multiply more quickly and can cluster together to form age spots.

AGING EVIDENCE

Changes in physiology

As all skin types age, skin loses volume and density, fine lines and wrinkles appear and changes in pigmentation can occur. Understanding and measuring these signs of ageing helps us to determine the condition of our skin. Skincare products should be selected to match skin type and address skin condition.

These are some of the visible signs of aging:

  • Dry skin
  • Dull and rough complexion
  • Fine lines
  • Deep wrinkles
  • Enlarged pores
  • Loss of firmness / sagging skin
  • Dark /age spots
  • Dark circles
  • Thin skin
  • Droopy eyelids

ACNE CAUSES AND TREATMENT

Acne is one of the main concerns for many people. Acne causes dark spots on the skin, scars, low self-esteem and depression on some people.

Acne appears when a pore in our skin clogs. This clog begins with dead skin cells. Normally, dead skin cells rise to surface of the pore, and the body sheds the cells. When the body starts to make lots of sebum (see-bum), oil that keeps our skin from drying out, the dead skin cells can stick together inside the pore.

Instead of rising to the surface, the cells become trapped inside the pore. Sometimes bacteria that live on our skin, also get inside the clogged pore. Inside the pore, the bacteria have a perfect environment for multiplying very quickly. With loads of bacteria inside, the pore becomes inflamed. If the inflammation goes deep into the skin, an acne cyst or nodule appears.

Active breakouts are frustrating enough, but the scars acne can leave behind can feel downright diabolical. The good news is that acne scars can be treated. However, before treatment can start you first have to get rid of any acne once and for all since new breakouts can lead to new acne scars.

 

The most common scars on the face are atrophic scars. A depressed scar sits below the surrounding skin. They’re formed when not enough collagen is made while the wound is healing. There are three types of atrophic scars:

 Boxcar scars are round or oval depressions with steep vertical sides. Wider than ice pick scars, boxcar scars give the skin an uneven, pitted appearance. These scars develop after an infection from a cyst or a deep inflamed blemish works its way to the surface.  The skin tissue is destroyed leaving these wide U-shaped scars.

 

Ice pick scars are deep holes that are small in size that are on the surface of the skin. These scars begin as comodones, also known as blackheads or whiteheads. Normally these are caused by acne. These acne scars are narrow, deep and depressed measuring 2mm or less in length. They appear vertically and are cylindrical in shape.

 

Rolling scars have smooth edges and look like tiny hills and valleys. These are also considered depressed acne scars. They often have a rough texture and a wavy or rippling appearance and they are the result of damage under the skin.

 

Dark spot is discoloration left behind after a zit has cleared and are purple, red, or brown marks.

 

Thanks to the revolutionary active ingredients patented by PBSerum, our acne and scar treatment products guarantee visible results in a very short time and are endorsed by numerous scientific studies* that certify their efficacy. You can find them with every product, as well as real photos of people before and after treatment.

 

All our products contain unique active ingredients, developed over years of biotechnological research in the cosmetic and pharmaceutical industry. PBSerum cosmeceutics are cosmetics with the efficacy of a drug, which greatly increases their efficacy and guarantees visible and rapid results, from the very first application.

 

All our facial products are based on patented active ingredients with proven efficacy. Their revolutionary Smart Peeling technology, thanks to the intelligent enzyme Keratinase KerA PB333, facilitates the action of the ingredients on very deep skin layers and guarantees effective, long-term results.

 

*Scientific studies carried out in Spain by Zurko Research and Clínica Med-Estetic.

 

 

PREVENTING AND REVERSING SKIN AGING

It is very important to keep our skin in its best condition. Although aging is inevitable, researches show that it is possible to slow and even reverse skin aging. The usage of anti-ageing products can help to reduce the appearance of wrinkles. PBSerum is the result of combined biotechnological and cosmetic research. The initials “pb” stand for Proteos Biotech, the company that develops the bio-active enzymes which are found in all our products. Many years of research in the pharmaceutical and cosmetic sector have enabled us to apply the latest technology to the world of beauty by creating PBSerum; cosmeceutics with immediate and long-term effects whose efficacy has been endorsed by numerous studies.

This is why in PBSerum we develop products of the highest quality and with proven results. Our products are cosmeceutics: cosmetics with the efficacy of a drug. Many years of ambitious biotechnological research in the cosmetic and pharmaceutical industry have allowed us to create unique active ingredients, patented and specifically designed to satisfy all your beauty needs, backed by numerous studies. Thanks to the innovative Smart Peeling technology, the intelligent enzymes of PBSerum penetrate easily into the deepest layers of the skin, guaranteeing long-term and highly effective results. Due to the process of lyophilization, the active ingredients remain stable for a long time. All our enzymes are 100% biological and therefore skin-friendly.

 

UNIQUE ACTIVE INGREDIENTS

  • Effective and pharmaceutically safe
  • Specifically designed to obtain the desired effects
  • Visible results in a very short time
  • Preservative -free thanks to lyophilization
  • High penetration capacity
  • 100% biological

 

 

PBSERUM ACTIVE INGREDIENTS: BIO-ACTIVE ENZYMES OF PROVEN EFFICACY.

Greater precision. Specifically developed for each pathology, our enzymes only act where the skin requires it.

 

Greater penetration. Their molecular composition is far smaller than that of traditional cosmetics, which allows the active ingredients to penetrate into the deepest layers of the skin.

 

Greater potency. Their much higher concentration enhances and prolongs the effect.

 

Greater lifespan. The lyophilization process all our active ingredients are submitted to is key for maintaining them stable and guaranteeing their efficacy for over 15 years without having to add preservatives or additives.

 

100% natural. As they are 100% biological, our enzymes are skin-friendly and do not produce irritations or side effects. They are the biological alternative to chemical products.

BIOGENETIC SERUM PRODUCTS AND HOW THEY WORK

Biogenetic Serum smartker Equilibrium

Reducing effect of facial imperfections and roughness.

RECOMMENDED FOR:

  • Dry skin, dilated pores
  • Hyperkeratosis
  • Skin irritation, skin disorders such as
  • Eczema, Dermatitis

BENEFITS:

  • Removes hardness, spots and roughness in the skin.
  • Repairs hyperkeratosis and scars.
  • Rejuvenate the skin.

 Biogenetic Serum smartker Multivit

Revitalizing effect for acne-prone skin

RECOMMENDED FOR:

  • Oily congested skin
  • Acne prone skin, all grades
  • Rosacea

BENEFITS:

  • Eliminates superficial fat
  • Roughness and spots reduction
  • Prevents dermatitis
  • Revitalizing effects

Biogenetic Serum smartker Radiant

Illuminator and anti-spots effect (melanin and erythemas)

RECOMMENDED FOR:

  • Any skin type with:
  • Hyper pigmentation, melasma, acne pigmentation
  • Post treatment pigmentation

BENEFITS:

  • Removes superficial blemishes
  • Lights up the skin
  • Increases skin´s firmness

Biogenetic Serum smartker Hyaluronic

Eliminating fine lines, rehydrating the skin

Anti-wrinkle and anti-aging effect

RECOMMENDED FOR:

  • Any skin type with: Crow feet, fine lines and wrinkles

BENEFITS:

  • Reduces wrinkles
  • Deeply hydrates the skin
  • Renews the skin

 Biogenetic Serum smartker Firmness

Address loss of elasticity and loss of firmness

Anti-aging, anti-fatigue and firmness effect

RECOMMENDED FOR:

  • Any skin type with:
  • Aging signs, wrinkles
  • Fatigue signs
  • Loss of firmness and elasticity

BENEFITS:

  • Firmness and skin tone improvement
  • Elasticity and brightness
  • Wrinkles reduction
  • Tightening and smoothing effect
  • Renews and moisturizes the skin deeply

 

APPLICATION

 DERMA ROLLER

A derma roller is a handheld roller-device covered in microneedles – needles ranging in length from 0.25 mm to 1.5m – which is rolled over the skin. This is done in order to create tiny punctures on the skin which triggers skin repair, leading to the creation of new collagen. A bodily protein made up of amino-acids, collagen is the part of the connective tissue that helps in firmness, strength, suppleness, and elasticity in skin.

Derma rolling is capable of exposing deeper layers of your skin to the benefits of various topical ointments, natural oils, treatments, and products, leading to greater effectiveness and therapeutic effect. In other words, after derma rolling, your skin will be more susceptible to the effects of anything that comes into contact with it, based on the increased exposure to the vital most layers of the skin. Therefore, PBSerum treatments enhance its effectiveness if applied in conjunction with derma rolling.

It’s also important to note that, you should remember not to panic to initial skin irritation and redness following derma rolling. This sort of a skin-reaction is to be expected in many cases – the skin, having been freshly ‘wounded’, needs to get worse before it gets better. The shorter the needle length, the lesser the irritation is likely to be, and results will generally vary depending on the person. Your skin is also likely to have increasingly diminished reactions to derma rolling, so keep at it and your redness and irritation is sure to wear down faster. Derma rolling should never be performed on any kind of open wound or skin blemish.

MASSAGING

Facial massage is the secret every skin pro relies on. Over 40 muscles make up the scaffolding of the face. Just like the muscles in the body, the more you move them, the more lifted, tightened and toned they become. When the aesthetician massages the face, it’s actually not solely to help you relax; those motions are also majorly good for your skin. The motions get the circulation going and can have positive short- and long-term effects, including decongesting oily areas and minimizing the appearance of fine lines.

By incorporating massage with fingers or tools like a jade roller, it increases the effectiveness of your facial treatments. The kneading strength can be adjusted by changing the angle of the roller, while the tool’s rounded tip can be used to target different acupuncture points.

On the epidermal level, light massage may encourage corneocyte shedding, increase epidermal proliferation and potentially increase skin thickness. Dermal tissue is also expected to benefit from increased blood flow and tensional forces in the dermis (induced by deeper mechanical stimulation), where dermal fibroblasts change phenotype and produce more collagen. Superficial massage, on the other hand, allows for the removal of interstitial fluid or lymphatic drainage. Benefits to consumer psychological wellbeing include relaxation and even temporary changes to the brainwave patterns. It has been proposed that repeated massage might have a cumulative effect with time.

 

Facialists have long known the power of touch and its ability to maximize skincare performance. More blood flow to the area increases collagen production, which gives a healthy and natural glow to the complexion. Massaging helps the product absorb more easily into the skin. It enhances the performance of topical skin care products. Simply gently stroking the face results in increased circulation, stimulating skin turnover and providing a healthy glow to any complexion, although product type should be selected based upon the individual skin type and condition.

Massaging the skin may influence the penetration of active ingredients. Studies measuring permeation rates reported that rubbing increased the flux of actives, reduced skin impedance and increased active ingredient retention. The concentration of the active ingredients in the product needs to be high enough to notice any effect from rubbing. Pressure on the skin also seems to increase the stratum corneum (SC) filling rate or increase follicular penetration, as seen for liposomal drug delivery systems. It has been reported that rubbing an active ingredient itself into the skin effectively enhances penetration, as opposed to simply rubbing the skin before the application.

Massaging the PBSerum has been shown to impact directly on the barrier function of the skin, thereby facilitating penetration, possibly by affecting the skin’s lipid structure. Therefore, there is an indication that rubbing promotes faster and deeper penetration into the skin’s layers.

 

BEFORE AND AFTER EXAMPLES

BEFORE AND AFTER

Biogenetic Serum smartker Equilibrium

BEFORE AND AFTER

Biogenetic Serum smartker Multivit

BEFORE AND AFTER

Biogenetic Serum smartker Radiant

BEFORE AND AFTER

Biogenetic Serum smartker Hyaluronic

BEFORE AND AFTER

 Biogenetic Serum smartker Firmness

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